On the mechanism of chloroquine resistance in Plasmodium falciparum Supporting Information

S1 PfCRT cannot be a channel for un-protonated chloroquine CQ S2 Membrane equilibrium equation S3 The expression for the Cellular Accumulation Ratio of chloroquine S4 Chloroquine concentration is equal in the external buffer and inside the erythrocyte S5 CAR expressions in the case of chloroquine-HM binding in linear regime (hypothesis H1) S6 Proof that [CQ]DV,D = [CQ]e in the hypothesis PfCRT is a channel for the protonated forms of chloroquine S7 Value of the channel permeability in the hypothesis PfCRT is a channel and [CQ:HM]DV = α[CQ]DV S8 Calculation of CARD in the hypothesis [CQ:HM]DV = α[CQ]DV (H1) and PfCRT is a non saturated active carrier (J2a) (main text, Figure 1, cells C3-5,5) S9 Detailed numerical calculation showing that chloroquine-HM binding in experiment C is not in the saturation region S10 Proof that eq. (18) (main text) is an increasing function of [H]DV in the interval 0 < pHDV < 9.15 S12 Calculation of CARD in saturated condition for chloroquine-HM binding and JPfCRT = λ[CQ ]DV (cells C5,1-4, Figure 1 of the main text]) S13 Calculations relating to each single hypothesis for the chloroquine:HM binding in saturated condition and JPfCRT = λ [CQ ]DV (cells C4,1-4, Figure 1 of the main text]) S14 Prediction for CARD in saturated condition for chloroquine-HM binding (H2) and JPfCRT = const (J2b) (cells C2,1-4,Figure 1, main text) _____________________________________________________________________